Journal: Cell Reports Methods

Article Title: Improved Sendai viral system for reprogramming to naive pluripotency

doi: 10.1016/j.crmeth.2022.100317

Figure Lengend Snippet:

Article Snippet: TIM-1 (HAVCR1) Antibody, anti-human, Biotin, RE-Afinity , Miltenyi Biotec , Cat#130-106-023, RRID: AB_2654152.

Techniques: Virus, Recombinant, Staining, SYBR Green Assay, DNA Methylation Assay, RNA Sequencing, Irradiation, Software

Journal: International Journal of Molecular Sciences

Article Title: Osthol Ameliorates Kidney Damage and Metabolic Syndrome Induced by a High-Fat/High-Sugar Diet

doi: 10.3390/ijms22052431

Figure Lengend Snippet: Effects of HF/HS diet and osthol administration on renal function and markers of renal damage. ( A ) Glomerular filtration rate (GFR), proteinuria, urinary nephrin excretion, N-acetyl-β-D-glucosaminidase (NAG) urine activity, urinary neutrophil gelatinase associated-lipocalin (NGAL) urinary excretion, and kidney injury molecule (KIM)-1 renal cortex expression. ( B ) Pro-inflammatory cytokine expression in the renal cortex (IL-1β and IL-6) and apoptosis biomarker expression (BAX and Bcl2). For Western blotting, 3 randomly selected samples per group were analyzed. Results are presented as the mean ± SD and analyzed by one-way ANOVA. Post hoc analysis was performed using Bonferroni’s test. a: p < 0.05 vs. Control; b: p < 0.05 vs. HF/HS; c: p < 0.05 vs. HF/HS + OT30).

Article Snippet: The antibodies used were as follows: fructokinase (Genetex, GTX109591, 1:5000 dilution), kidney injury molecule (KIM-1) (Genetex, GTX85067, 1:3000 dilution), fatty acid synthase (Genetex, GTX 13550, 1:2500 dilution), xanthine oxidase (Santacruz Biotechnology, sc398548 1:1000 dilution), Nox-4 (Novus Biologicals, NB-110-58851, 1:1000 dilution), nephrin (Genetex, GTX 31654, 1:10,000 dilution), IL-1β (Santacruz Biotechnology, sc-1250, 1:1000 dilution), IL-6 (Santacruz Biotecnology, sc-57315, 1:3000 dilution), phospho-AMPK Thr 172 (Cell Signaling, 40H9, 1:2000 dilution), Sirt-1 (Cell Signaling, D739, 1:2500 dilution), SREBP1 (Genetex, 79299, 1:3000 dilution), Nrf2 (Genetex, 103322, 1:1500 dilution), superoxide dismutase (Santacruz Biotechnology, sc101523, 1:1000 dilution), catalase (Santacruz Biotechnology, sc271358, 1:1500 dilution), glutathione peroxidase (Santacruz Biotechnology, sc22145, 1:1000 dilution), β-actin (Genetex, 109639, 1:10,000 dilution), proliferation cell nuclear antigen (PCNA; GTX100539, 1:2000 dilution), and neutrophil gelatinase associated-lipocalin (NGAL, Santacruz Biotechnology, sc-515876, 1:10,000 dilution).

Techniques: Filtration, Activity Assay, Expressing, Biomarker Assay, Western Blot

Journal: Molecular Medicine Reports

Article Title: Urinary kidney injury molecule-1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method

doi: 10.3892/mmr.2017.6103

Figure Lengend Snippet: Evaluation of KIM-1, α-SMA and vimentin expression in Sham, UUO1, UUO3 and UUO7 groups by scoring of immunohistochemical assays.

Article Snippet: Immunohistochemistry (IHC) kits for KIM-1, α-SMA and vimentin were obtained from BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Expressing, Immunohistochemical staining

Journal: Molecular Medicine Reports

Article Title: Urinary kidney injury molecule-1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method

doi: 10.3892/mmr.2017.6103

Figure Lengend Snippet: Comparison of KIM-1, α-SMA and vimentin expression in kidney tissues of rats at 1 (UUO1), 3 (UUO3) and 7 (UUO7) days following UUO. Representative immunohistochemistry results of KIM-1, α-SMA and vimentin protein expression (magnification,

Article Snippet: Immunohistochemistry (IHC) kits for KIM-1, α-SMA and vimentin were obtained from BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Expressing, Immunohistochemistry, Staining